A 2-month-old infant presented with immature teratoma, containing tissues derived from extoderm, endoderm and mesoderm, and detected at trigone in lateral ventricle.
The size of teratoma was large at discovery that an exact localization of the origin was not possible.
Karyotyping was performed on this tetratoma by G-banding from primary culture to third passage. Cytogenetic characteristics of this immature teratoma were hypodiploid stem line(45, XX, -22) with a related normal diploid side line.
In addition to chromosome 22, other chromosome including gonsome were lost randomly.
Hypotetraploid and trisomy 12 were observed only 3 cells and 2 cells from 48 cells analysed respectively.
We wish to recommend that whenever the culture method is used chromosome analysis should be performed on cells from primary cultures as soon as possible, because the rapid growth of normal cells in vitro from immature tetratomas was observed in
passage
1 to passage 3.
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